Hotspot

ndhC-trnV-UAC

LSC · 50764-51748 · 985 bp

Back to Scutellaria

Pi 0.0217
MarkerSeek score 67.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
985
variable_sites
213
pi
0.0217
unique_haplotype_count
125
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 33.3 55.0 56.3 58.1 550-1128 bp 1.0 52.0
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAAGACTTG CCGAGAAAGTCTACGGTTCG 34.8 55.0 54.9 58.1 550-1128 bp 1.0 52.0
ndhC-trnV-UAC_p3 ATGCCAAAATAGGAATAAGACTTGA CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 551-1129 bp 1.0 52.0
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAAGACTTGAT CCGAGAAAGTCTACGGTTCG 32.0 55.0 56.8 58.1 550-1128 bp 1.0 52.0
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAAGACTTGA GTTCGAGTCCGTATAGCCCT 33.3 55.0 56.3 58.7 535-1113 bp 0.994382 51.8