Hotspot

trnT-UGU-trnL-UAA

LSC · 46830-47509 · 680 bp

Back to Scutellaria

Pi 0.0263

MarkerSeek score 71.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 219
pi: 0.0263
unique_haplotype_count: 119
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GTCTACCGATTTCGCCACAT	55.0	50.0	57.9	58.1	673-757 bp	1.0	90.4
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	GTTTCCCACATCAAGGCTCA	55.0	50.0	57.9	58.1	712-796 bp	1.0	90.2
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	GTCTACCGATTTCGCCACAT	50.0	50.0	57.8	58.1	705-789 bp	1.0	89.6
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	GTTTCCCACATCAAGGCTCA	50.0	50.0	57.8	58.1	744-828 bp	1.0	89.4
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TCTACCGATTTCGCCACATC	55.0	50.0	57.9	57.8	672-756 bp	1.0	89.2