Hotspot

ycf3-trnS-GGA

LSC · 44565-45447 · 883 bp

Back to Scutellaria

Pi 0.0166

MarkerSeek score 67.50

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 883
variable_sites: 170
pi: 0.0166
unique_haplotype_count: 114
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TCTCCTGAGGTTGTCGGAAT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.1	58.1	502-1066 bp	1.0	90.8
ycf3-trnS-GGA_p2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	58.1	58.2	523-1087 bp	1.0	90.3
ycf3-trnS-GGA_p3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.1	57.8	473-1037 bp	1.0	90.0
ycf3-trnS-GGA_p4	TTCTCCTGAGGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	503-1067 bp	1.0	88.9
ycf3-trnS-GGA_p5	TTTCTCCTGAGGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.3	58.1	504-1068 bp	1.0	88.9