Hotspot

petN-psbM

LSC · 29047-30062 · 1016 bp

Back to Scutellaria

Pi 0.0248

MarkerSeek score 65.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1016
variable_sites: 243
pi: 0.0248
unique_haplotype_count: 133
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	CAGTCCCGACGGATCAAAAT	50.0	50.0	58.0	58.0	1505-1810 bp	1.0	91.8
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	CAGTCCCGACGGATCAAAAT	50.0	50.0	58.0	58.0	1503-1808 bp	1.0	91.8
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	GTCCCGACGGATCAAAATCA	50.0	50.0	58.0	58.0	1503-1808 bp	0.994382	91.6
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	GTCCCGACGGATCAAAATCA	50.0	50.0	58.0	58.0	1501-1806 bp	0.994382	91.6
petN-psbM_p5	TCGTAGTGTGGGGAAGAAGT	AATTTTCTGCTCGATCCCCC	50.0	50.0	58.0	57.9	1602-1907 bp	0.617978	76.2