Hotspot

rps16-trnQ-UUG

LSC · 6320-7064 · 745 bp

Back to Scutellaria

Pi 0.0191

MarkerSeek score 68.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 212
pi: 0.0191
unique_haplotype_count: 135
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	723-1275 bp	1.0	90.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	706-1258 bp	1.0	90.3
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	724-1276 bp	1.0	89.7
rps16-trnQ-UUG_p4	AGCACCCTCATTCCTAGACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	772-1324 bp	1.0	89.4
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	720-1272 bp	1.0	88.4