Hotspot

trnK-UUU-rps16

LSC · 4256-5156 · 901 bp

Back to Scutellaria

Pi 0.0335
MarkerSeek score 72.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
901
variable_sites
245
pi
0.0335
unique_haplotype_count
149
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCCTTTCAGGATCAGTCGTG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1392-1866 bp 0.926966 88.9
trnK-UUU-rps16_p2 CCTTTCAGGATCAGTCGTGG AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1391-1865 bp 0.926966 88.9
trnK-UUU-rps16_p3 CCCTTTCAGGATCAGTCGTG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 882-1285 bp 0.853933 85.6
trnK-UUU-rps16_p4 CCTTTCAGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 881-1284 bp 0.853933 85.6
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 882-1285 bp 0.853933 85.6