Hotspot

trnR-ACG-trnN-GUU

IRb · 91348-91895 · 548 bp

Back to Scurrula

Pi 0.0444
MarkerSeek score 87.40
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
548
variable_sites
69
pi
0.0444
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 GGTACTCCCGGGAACAGATA TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.9 58.2 681-724 bp 1.0 90.0
trnR-ACG-trnN-GUU_p2 GGTACTCCCGGGAACAGATA GCTGTTAACTGACTGGTCGT 55.0 50.0 57.9 57.8 694-737 bp 1.0 89.9
trnR-ACG-trnN-GUU_p3 GGTACTCCCGGGAACAGATA CTGGTCGTAGGTTCGAATCC 55.0 55.0 57.9 57.8 682-725 bp 1.0 89.6
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG GCTGTTAACTGACTGGTCGT 55.0 50.0 57.8 57.8 646-689 bp 1.0 89.1
trnR-ACG-trnN-GUU_p5 GGTACTCCCGGGAACAGATA CTGTTCCTCAGTAGCTCAGC 55.0 55.0 57.9 57.7 727-770 bp 1.0 88.4