Hotspot

rbcL-accD

LSC · 45568-46114 · 547 bp

Back to Scurrula

Pi 0.0569

MarkerSeek score 84.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 95
pi: 0.0569
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCCGTAGCTAATCGAGTAGC	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.0	58.1	766-827 bp	1.0	90.8
rbcL-accD_p2	GCTCTGACCGAGATCTTTGG	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.1	58.1	850-911 bp	1.0	90.3
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CCGTGCTCAAACTCTTCCTT	50.0	50.0	57.9	58.1	646-707 bp	1.0	90.2
rbcL-accD_p4	GCCTTACGTATGTCTGGTGG	CCGTGCTCAAACTCTTCCTT	55.0	50.0	58.1	58.1	1066-1127 bp	1.0	89.1
rbcL-accD_p5	AAAAGACCGAAGTAGGGGGA	CCGTGCTCAAACTCTTCCTT	50.0	50.0	58.0	58.1	950-1011 bp	0.857143	84.0