Pi
0.0569
MarkerSeek score
79.40
Primer available
yes
Label
rps4-trnT-UGU
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps4-trnT-UGU_p1 | CGAAGCCATACCCAATCGAA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1283-1383 bp | 1.0 | 91.5 |
| rps4-trnT-UGU_p2 | CAATTCCGCCTGCAAAGATG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1457-1557 bp | 1.0 | 91.3 |
| rps4-trnT-UGU_p3 | TGTTCATTCTTCGCCGTGAT | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 57.9 | 58.0 | 1402-1502 bp | 1.0 | 91.2 |
| rps4-trnT-UGU_p4 | ATTGTCGGGCTCCAGAGATA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1319-1405 bp | 0.857143 | 85.5 |
| rps4-trnT-UGU_p5 | ATTTGACCTGTTGGCCCTTT | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 57.9 | 58.0 | 1222-1285 bp | 0.857143 | 83.7 |