Hotspot

ndhF

SSC · 108708-110939 · 2232 bp

Back to Scrophularia

Pi 0.00987

MarkerSeek score 57.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 183
pi: 0.00987
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCAGAATACCGTCACCCATT	AGGTAAGATCCGGTGAATCG	50.0	50.0	58.0	56.8	2504-2541 bp	0.9375	77.6
ndhF_p2	AGGGTGGAAAGTGAGGAAGA	AGGTAAGATCCGGTGAATCG	50.0	50.0	57.9	56.8	2445-2482 bp	0.9375	77.1
ndhF_p3	CGGAAGAAAGGGTGGAAAGT	AGGTAAGATCCGGTGAATCG	50.0	50.0	57.7	56.8	2453-2490 bp	0.9375	75.9
ndhF_p4	ACGGAAGAAAGGGTGGAAAG	AGGTAAGATCCGGTGAATCG	50.0	50.0	57.7	56.8	2454-2491 bp	0.9375	75.8
ndhF_p5	GCAGAATACCGTCACCCATT	AAGGTAAGATCCGGTGAATCG	50.0	47.6	58.0	57.5	2505-2542 bp	0.9375	75.4