Hotspot

rps15-ycf1

SSC · 121684-122078 · 395 bp

Back to Scrophularia

Pi 0.0112

MarkerSeek score 54.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 395
variable_sites: 35
pi: 0.0112
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGAGTCATTCTTCCCATGCA	AAAACAGCAGCCAAGTTTCG	50.0	45.0	58.0	57.8	928-972 bp	0.96875	86.5
rps15-ycf1_p2	CCCGAGTCATTCTTCCCATG	AAAACAGCAGCCAAGTTTCG	55.0	45.0	58.0	57.8	930-974 bp	0.96875	86.4
rps15-ycf1_p3	TCCCATGCATTGCTGAAAGT	AAAACAGCAGCCAAGTTTCG	45.0	45.0	58.1	57.8	917-961 bp	0.96875	86.3
rps15-ycf1_p4	AACACCATGCATTGATGGGT	AAAACAGCAGCCAAGTTTCG	45.0	45.0	58.1	57.8	1014-1058 bp	0.96875	85.6
rps15-ycf1_p5	CGAGTCATTCTTCCCATGCA	GAACCCATTCGTCTGTCTGG	50.0	55.0	58.0	58.3	1253-1297 bp	0.875	81.3