Hotspot

petA-psbJ

LSC · 62518-63557 · 1040 bp

Back to Scrophularia

Pi 0.0144

MarkerSeek score 68.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1040
variable_sites: 97
pi: 0.0144
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGAT	50.0	50.0	58.2	56.2	917-1225 bp	1.0	74.8
petA-psbJ_p2	AGAAGGTTCAATTGGCCGAA	GCCGATACTACTGGAAGGAT	45.0	50.0	57.7	56.2	916-1224 bp	1.0	74.0
petA-psbJ_p3	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGATT	50.0	47.6	58.2	57.0	917-1225 bp	1.0	72.9
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	GCCGATACTACTGGAAGGATTC	50.0	50.0	58.2	58.1	917-1225 bp	1.0	72.5
petA-psbJ_p5	AGAAGGTTCAATTGGCCGAA	GCCGATACTACTGGAAGGATT	45.0	47.6	57.7	57.0	916-1224 bp	1.0	72.1