Hotspot

ycf3-trnS-GGA

LSC · 43978-44625 · 648 bp

Back to Scrophularia

Pi 0.0118

MarkerSeek score 70.60

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 55
pi: 0.0118
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	496-1055 bp	1.0	80.6
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.1	58.2	495-1054 bp	1.0	80.6
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	446-1005 bp	1.0	80.4
ycf3-trnS-GGA_p4	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	475-1034 bp	0.9375	78.3
ycf3-trnS-GGA_p5	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	474-1033 bp	0.9375	78.3