Hotspot

petN-psbM

LSC · 28341-29273 · 933 bp

Back to Scrophularia

Pi 0.0113

MarkerSeek score 67.40

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 79
pi: 0.0113
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	669-1289 bp	1.0	78.5
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	667-1287 bp	1.0	78.5
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.9	57.5	666-1286 bp	1.0	77.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.2	57.5	721-1341 bp	1.0	77.0
petN-psbM_p5	GTAGTGTGGGGAAGAAGTGG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.5	57.5	665-1285 bp	1.0	74.8