Hotspot

trnC-GCA-petN

LSC · 27400-28250 · 851 bp

Back to Scrophularia

Pi 0.0114

MarkerSeek score 67.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 851
variable_sites: 69
pi: 0.0114
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	943-975 bp	1.0	88.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	941-973 bp	1.0	88.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CACTTCTTCCCCACACTACG	50.0	55.0	58.1	57.9	944-976 bp	1.0	88.1
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	889-921 bp	1.0	88.1
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCTAGAGCCCACTTCTTCCC	50.0	55.0	58.1	58.1	953-985 bp	1.0	87.6