Hotspot

rpoB-trnC-GCA

LSC · 26209-27328 · 1120 bp

Back to Scrophularia

Pi 0.0117

MarkerSeek score 69.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1120
variable_sites: 93
pi: 0.0117
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	978-1221 bp	1.0	87.8
rpoB-trnC-GCA_p2	CATTGCCTCATTTGCATCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.8	58.4	960-1203 bp	1.0	85.8
rpoB-trnC-GCA_p3	ATTGCCTCATTTGCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.9	58.1	977-1220 bp	1.0	82.7
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGTTGATCAGGCGACACC	50.0	50.0	57.8	58.8	997-1240 bp	1.0	82.3
rpoB-trnC-GCA_p5	ACATTGCCTCATTTGCATCC	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.0	58.1	979-1222 bp	1.0	81.9