Hotspot

psbK-psbI

LSC · 6903-7274 · 372 bp

Back to Scrophularia

Pi 0.0152

MarkerSeek score 67.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 45
pi: 0.0152
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	GGATTACGTCCCGGATCATT	45.0	50.0	58.1	57.2	435-502 bp	1.0	84.3
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGTCCGGGATTAC	50.0	50.0	57.9	57.3	443-510 bp	0.96875	84.3
psbK-psbI_p3	CTTTTGTTTGGCAAGCTGCT	TTCTTCACGTCCGGGATTAC	45.0	50.0	58.1	57.3	448-515 bp	0.96875	84.2
psbK-psbI_p4	TATGCCAGTCATCCCTCTGT	TTCTTCACGTCCGGGATTAC	50.0	50.0	57.8	57.3	483-550 bp	0.96875	82.8
psbK-psbI_p5	GCCAGTCATCCCTCTGTTTT	TTCTTCACGTCCGGGATTAC	50.0	50.0	57.8	57.3	480-547 bp	0.96875	82.6