Hotspot

ycf1

SSC · 122471-127501 · 5031 bp

Back to Scorzonera

Pi 0.0122

MarkerSeek score 52.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5031
variable_sites: 61
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTCAGAAGAGGTTTTTCAAACAGA	TGGTACTTTCTTCTTTCTTACCTGT	37.5	36.0	57.6	57.9	219-234 bp	1.0	46.1
ycf1_p2	GTCAGAAGAGGTTTTTCAAACAGA	AGCTGTTATGTTTGGTACTTTCT	37.5	34.8	57.6	56.1	231-246 bp	1.0	46.0
ycf1_p3	CAGAAGAGGTTTTTCAAACAGAGA	AGCTGTTATGTTTGGTACTTTCT	37.5	34.8	57.3	56.1	229-244 bp	1.0	46.0
ycf1_p4	TCAGAAGAGGTTTTTCAAACAGAG	AGCTGTTATGTTTGGTACTTTCT	37.5	34.8	57.3	56.1	230-245 bp	1.0	46.0
ycf1_p5	TCAGAAGAGGTTTTTCAAACAGA	AGCTGTTATGTTTGGTACTTTCT	34.8	34.8	56.3	56.1	230-245 bp	1.0	46.0