Hotspot

rpoC2

LSC · 19021-23166 · 4146 bp

Back to Scorzonera

Pi 0.00651

MarkerSeek score 42.70

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4146
variable_sites: 27
pi: 0.00651
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TTGCCCGGAGAACTAATTGG	AAGGTGTTTTGAAGCCGCTA	50.0	45.0	57.9	58.0	265 bp	1.0	80.0
rpoC2_p2	TTGCCCGGAGAACTAATTGG	GTTTTGAAGCCGCTACCAAC	50.0	50.0	57.9	58.0	260 bp	1.0	79.9
rpoC2_p3	TTGCCCGGAGAACTAATTGG	AGGTTCGCTTGAAGGTGTTT	50.0	45.0	57.9	57.9	276 bp	1.0	79.8
rpoC2_p4	TTGCCCGGAGAACTAATTGG	CCCCCAGAACAACGTTTTCT	50.0	50.0	57.9	58.3	229 bp	1.0	77.9
rpoC2_p5	TTGCCCGGAGAACTAATTGG	TTTTGAAGCCGCTACCAACA	50.0	45.0	57.9	58.3	259 bp	1.0	77.7