Hotspot

rps7-rps12

IRb · 96559-96611 · 53 bp

Back to Scorzonera

Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTCTTCTGCAGTACCTCGAC	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.0	57.7	149 bp	1.0	69.7
rps7-rps12_p2	TCTTCTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	55.0	55.0	59.2	57.7	148 bp	1.0	68.2
rps7-rps12_p3	TTCTTCTGCAGTACCTCGAC	TATGTACGGCTTGGAGGGAG	50.0	55.0	57.0	58.6	156 bp	1.0	67.7
rps7-rps12_p4	TTTCTTCTGCAGTACCTCGAC	GGCTTGGAGGGAGATCTTTC	47.6	55.0	57.7	57.7	150 bp	1.0	67.4
rps7-rps12_p5	CTTCTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	57.9	55.0	57.7	57.7	147 bp	1.0	67.3