Hotspot

rpl16-rps3

LSC · 81872-82037 · 166 bp

Back to Scorzonera

Pi 0.0316

MarkerSeek score 63.70

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 5
pi: 0.0316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAAAACCGACGAGTCACACA	GAGGGTAGGGTTCCACTACA	45.0	55.0	58.0	57.8	303-311 bp	1.0	81.3
rpl16-rps3_p2	CCGACGAGTCACACACTAAG	GAGGGTAGGGTTCCACTACA	55.0	55.0	58.0	57.8	298-306 bp	1.0	81.2
rpl16-rps3_p3	AAAAACCGACGAGTCACACA	AGAGGGTAGGGTTCCACTAC	45.0	55.0	58.0	57.5	304-312 bp	1.0	78.9
rpl16-rps3_p4	CCGACGAGTCACACACTAAG	AGAGGGTAGGGTTCCACTAC	55.0	55.0	58.0	57.5	299-307 bp	1.0	78.8
rpl16-rps3_p5	GACGAGTCACACACTAAGCA	GAGGGTAGGGTTCCACTACA	50.0	55.0	57.6	57.8	296-304 bp	1.0	78.2