Hotspot

rpl14-rpl16

LSC · 80337-80463 · 127 bp

Back to Scorzonera

Pi 0.0242

MarkerSeek score 68.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 3
pi: 0.0242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ACACATCAATTCTCGAGCCC	GCTATTTCAATCGCAGCGTC	50.0	50.0	58.0	58.0	247-250 bp	1.0	81.6
rpl14-rpl16_p2	ACACATCAATTCTCGAGCCC	TATTTCAATCGCAGCGTCCA	50.0	45.0	58.0	58.0	245-248 bp	1.0	81.6
rpl14-rpl16_p3	TCGACGATTGCTAGCTCCTA	GCTATTTCAATCGCAGCGTC	50.0	50.0	58.0	58.0	277-280 bp	1.0	81.3
rpl14-rpl16_p4	TCGACGATTGCTAGCTCCTA	TATTTCAATCGCAGCGTCCA	50.0	45.0	58.0	58.0	275-278 bp	1.0	81.3
rpl14-rpl16_p5	CCCGCTGTTATCTGCTACAT	GCTATTTCAATCGCAGCGTC	50.0	50.0	57.5	58.0	229-232 bp	1.0	77.7