Hotspot

ndhC-trnV-UAC

LSC · 50143-51384 · 1242 bp

Back to Scorzonera

Pi 0.00684
MarkerSeek score 67.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1242
variable_sites
8
pi
0.00684
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1327-1387 bp 1.0 80.4
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1329-1389 bp 1.0 79.5
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 1332-1392 bp 1.0 79.5
ndhC-trnV-UAC_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1326-1386 bp 1.0 77.6
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 1328-1388 bp 1.0 77.3