Hotspot

ycf3-trnS

LSC · 43883-44823 · 941 bp

Back to Scorzonera

Pi 0.016

MarkerSeek score 67.60

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 941
variable_sites: 15
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CATTTATCCGCGATCTAGGCA	ACGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	58.2	1051-1067 bp	1.0	72.1
ycf3-trnS_p2	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	1001-1017 bp	1.0	71.8
ycf3-trnS_p3	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	1039-1055 bp	1.0	71.5
ycf3-trnS_p4	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	1003-1019 bp	1.0	71.3
ycf3-trnS_p5	CATTTATCCGCGATCTAGGCA	CCTCGGTAAGCAAAAGCCTA	47.6	50.0	58.0	57.6	1029-1045 bp	1.0	70.1