Hotspot

trnY-GUA-trnE-UUC

LSC · 11837-12028 · 192 bp

Back to Scorzonera

Pi 0.0312

MarkerSeek score 63.60

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 6
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	AAAACGAGGGTTCAAGTCCC	55.0	50.0	58.4	58.0	265 bp	1.0	78.8
trnY-GUA-trnE-UUC_p2	ACGAATTTACAGTCCGTCCC	AAAACGAGGGTTCAAGTCCC	50.0	50.0	57.6	58.0	266 bp	1.0	78.6
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	CGGAGAAAACGAGGGTTCAA	55.0	50.0	58.4	58.1	270 bp	1.0	78.0
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	CCCCTATGGTCCAGTGGTTA	55.0	55.0	58.4	58.1	306 bp	1.0	77.9
trnY-GUA-trnE-UUC_p5	CGAATTTACAGTCCGTCCCC	TTTCACGGAGAAAACGAGGG	55.0	50.0	58.4	58.1	275 bp	1.0	77.9