Hotspot

psbM-trnD-GUC

LSC · 10911-11574 · 664 bp

Back to Scorzonera

Pi 0.0151
MarkerSeek score 58.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
664
variable_sites
10
pi
0.0151
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TTCAATAGGCAAGAGCACCG 39.1 50.0 57.6 58.3 779-781 bp 1.0 51.7
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TTCAATAGGCAAGAGCACCG 40.0 50.0 54.5 58.3 773-775 bp 1.0 51.3
psbM-trnD-GUC_p3 ACTAAAATGAACAGTGCAGTAGC TTCAATAGGCAAGAGCACCG 39.1 50.0 57.4 58.3 776-778 bp 1.0 50.3
psbM-trnD-GUC_p4 AGGAACTAAAATGAACAGTGCAG TTCAATAGGCAAGAGCACCG 39.1 50.0 57.3 58.3 780-782 bp 1.0 49.7
psbM-trnD-GUC_p5 AGGAACTAAAATGAACAGTGCA TTCAATAGGCAAGAGCACCG 36.4 50.0 56.2 58.3 780-782 bp 1.0 48.8