Hotspot

rps16

LSC · 4651-5711 · 1061 bp

Back to Scorpiothyrsus

Pi 0.0132

MarkerSeek score 57.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1061
variable_sites: 21
pi: 0.0132
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AAAATTTCCCCCTTATCCTTT	ATACCAGTTAAAACCCGGGG	33.3	50.0	52.8	57.2	1115-1145 bp	1.0	42.0
rps16_p2	AAATTTCCCCCTTATCCTTTT	ATACCAGTTAAAACCCGGGG	33.3	50.0	52.8	57.2	1114-1144 bp	1.0	42.0
rps16_p3	AATTTCCCCCTTATCCTTTTT	ATACCAGTTAAAACCCGGGG	33.3	50.0	52.8	57.2	1113-1143 bp	1.0	42.0
rps16_p4	AGGGGGATCTACTAGAAAAA	ATACCAGTTAAAACCCGGGG	40.0	50.0	52.2	57.2	1152-1182 bp	1.0	41.9
rps16_p5	AGGGGGATCTACTAGAAAAA	ACATACCAGTTAAAACCCGGG	40.0	47.6	52.2	57.9	1154-1184 bp	1.0	41.9