Hotspot

psbA

LSC · 306-1367 · 1062 bp

Back to Scorpiothyrsus

Pi 0.00314

MarkerSeek score 34.50

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 5
pi: 0.00314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AACACAAAACTACTGGCCGT	55.0	45.0	58.1	58.0	2034-2042 bp	1.0	80.3
psbA_p2	GATCCACTTGGCTACATCCG	ACAAAACTACTGGCCGTGTT	55.0	45.0	58.1	58.0	2031-2039 bp	1.0	80.3
psbA_p3	CAATCCACTGCCTTGATCCA	AACACAAAACTACTGGCCGT	50.0	45.0	57.9	58.0	2048-2056 bp	1.0	80.2
psbA_p4	CAATCCACTGCCTTGATCCA	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2045-2053 bp	1.0	80.2
psbA_p5	AATCCACTGCCTTGATCCAC	ACAAAACTACTGGCCGTGTT	50.0	45.0	57.9	58.0	2044-2052 bp	1.0	80.2