Hotspot

ndhF-rpl32

SSC · 114469-115356 · 888 bp

Back to Scorpiothyrsus

Pi 0.0359

MarkerSeek score 72.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 47
pi: 0.0359
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1405-1422 bp	1.0	85.4
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1406-1423 bp	1.0	85.3
ndhF-rpl32_p3	TGGCAACTGCACCTGTAAAT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	2071-2088 bp	1.0	84.0
ndhF-rpl32_p4	TGGCAACTGCACCTGTAAAT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	2072-2089 bp	1.0	84.0
ndhF-rpl32_p5	CGCCTACACGATTCGTTACA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1909-1926 bp	1.0	83.5