Hotspot

rpl33-rps18

LSC · 68617-68840 · 224 bp

Back to Scorpiothyrsus

Pi 0.0239

MarkerSeek score 57.20

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 8
pi: 0.0239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGATTCACGGGGAGATAAAGA	GCAAACGCCTACGAAAAGAT	47.6	45.0	57.3	57.1	304-308 bp	1.0	62.2
rpl33-rps18_p2	CGATTCACGGGGAGATAAAGA	GGCAAACGCCTACGAAAAGA	47.6	50.0	57.3	59.1	305-309 bp	1.0	60.3
rpl33-rps18_p3	CGATTCACGGGGAGATAAAGAA	GCAAACGCCTACGAAAAGAT	45.5	45.0	57.9	57.1	304-308 bp	1.0	59.5
rpl33-rps18_p4	CGATTCACGGGGAGATAAAGA	GCAAACGCCTACGAAAAGATC	47.6	47.6	57.3	58.2	304-308 bp	1.0	59.5
rpl33-rps18_p5	CGATTCACGGGGAGATAAAGA	GGCAAACGCCTACGAAAAG	47.6	52.6	57.3	57.6	305-309 bp	1.0	58.3