Hotspot

rbcL-accD

LSC · 57363-57985 · 623 bp

Back to Scorpiothyrsus

Pi 0.0194

MarkerSeek score 73.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 18
pi: 0.0194
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	695-711 bp	1.0	81.5
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ATCCTCACTTCCACGAGTGT	50.0	50.0	57.9	58.4	784-800 bp	1.0	80.2
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	ACATCGAATTAAACCGCCGT	55.0	45.0	57.7	58.3	711-727 bp	1.0	79.9
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	CACTTCCACGAGTGTCTTCA	50.0	50.0	57.9	57.5	779-795 bp	1.0	79.1
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	ATCCTCACTTCCACGAGTGT	55.0	50.0	57.7	58.4	800-816 bp	1.0	78.7