Hotspot

ndhC-trnV-UAC

LSC · 51405-52075 · 671 bp

Back to Scorpiothyrsus

Pi 0.014

MarkerSeek score 65.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 13
pi: 0.014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGACCTTTACTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.7	57.9	802-857 bp	1.0	81.9
ndhC-trnV-UAC_p2	AATCGGGGCTAAAATTCCGG	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.3	57.9	791-846 bp	1.0	81.4
ndhC-trnV-UAC_p3	GGACCTTTACTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.7	58.3	803-858 bp	1.0	80.0
ndhC-trnV-UAC_p4	CGGGGCTAAAATTCCGGAAA	GGTTCGAATCCGTATAGCCC	50.0	55.0	58.5	57.9	788-843 bp	1.0	79.7
ndhC-trnV-UAC_p5	AATCGGGGCTAAAATTCCGG	CGGTTCGAATCCGTATAGCC	50.0	55.0	58.3	58.3	792-847 bp	1.0	79.6