Hotspot

trnF-GAA-ndhJ

LSC · 48807-49682 · 876 bp

Back to Scorpiothyrsus

Pi 0.0114

MarkerSeek score 54.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 876
variable_sites: 15
pi: 0.0114
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	931-954 bp	1.0	41.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TTGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.6	932-955 bp	1.0	41.9
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	58.0	53.8	967-990 bp	1.0	41.8
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TGAAATACAAGATGCTCATTGA	55.0	31.8	57.9	53.8	955-978 bp	1.0	41.8
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TTGAAATACAAGATGCTCATTGA	55.0	30.4	58.0	54.6	968-991 bp	1.0	41.8