Hotspot

trnfM-CAU-rps14

LSC · 37356-37527 · 172 bp

Back to Scorpiothyrsus

Pi 0.0465

MarkerSeek score 74.00

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 12
pi: 0.0465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	334-335 bp	1.0	82.8
trnfM-CAU-rps14_p2	TTTGAACCCGTGACCTCAAG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	351-352 bp	1.0	82.5
trnfM-CAU-rps14_p3	AAGGTTATGAGCCTTGCGAG	GGCTTTCCGGACACATACTT	50.0	50.0	58.0	57.9	288-289 bp	1.0	82.5
trnfM-CAU-rps14_p4	AAGGTTATGAGCCTTGCGAG	ATCGAGACTTTGGGCTTTCC	50.0	50.0	58.0	57.9	300-301 bp	1.0	82.3
trnfM-CAU-rps14_p5	ACAGGATTTGAACCCGTGAC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.1	58.0	357-358 bp	1.0	81.9