Hotspot

rpoB-trnC-GCA

LSC · 27122-28475 · 1354 bp

Back to Scorpiothyrsus

Pi 0.0163
MarkerSeek score 61.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1354
variable_sites
33
pi
0.0163
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCACTTCCATCCACCAGCAT CCGGATTTGAACTGGGGAAA 50.0 50.0 59.0 58.1 1436-1470 bp 1.0 74.9
rpoB-trnC-GCA_p2 TTCCCTCACTTCCATCCACC CCGGATTTGAACTGGGGAAA 55.0 50.0 59.0 58.1 1441-1475 bp 1.0 74.8
rpoB-trnC-GCA_p3 GACATTCCCTCACTTCCATCC CCGGATTTGAACTGGGGAAA 52.4 50.0 58.1 58.1 1445-1479 bp 1.0 74.3
rpoB-trnC-GCA_p4 CATTCCCTCACTTCCATCCA CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1443-1477 bp 1.0 74.0
rpoB-trnC-GCA_p5 ATTCCCTCACTTCCATCCAC CCGGATTTGAACTGGGGAAA 50.0 50.0 56.9 58.1 1442-1476 bp 1.0 74.0