Hotspot

atpF-atpH

LSC · 13094-13615 · 522 bp

Back to Scorpiothyrsus

Pi 0.0128

MarkerSeek score 66.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 10
pi: 0.0128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GGCCCAAGGAAAGGAAAGAA	GGATTGGTTGTCGCTTTAGC	50.0	50.0	58.0	57.5	599-607 bp	1.0	78.0
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGATTGGTTGTCGCTTTAGC	50.0	50.0	57.9	57.5	631-639 bp	1.0	77.0
atpF-atpH_p3	GGCCCAAGGAAAGGAAAGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	664-672 bp	1.0	76.6
atpF-atpH_p4	GGCCCAAGGAAAGGAAAGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	665-673 bp	1.0	76.4
atpF-atpH_p5	GGCCCAAGGAAAGGAAAGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	666-674 bp	1.0	76.4