Hotspot

trnS-GCU-trnR-UCU

LSC · 7961-9779 · 1819 bp

Back to Scorpiothyrsus

Pi 0.0134

MarkerSeek score 65.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1819
variable_sites: 36
pi: 0.0134
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1933-1952 bp	1.0	70.9
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAACTTGCGTCCAATAGGA	50.0	45.0	58.0	56.6	1932-1951 bp	1.0	70.9
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1940-1959 bp	1.0	69.0
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	ACATAGAAGAACTTGCGTCCA	50.0	42.9	58.0	57.3	1939-1958 bp	1.0	69.0
trnS-GCU-trnR-UCU_p5	CAATCCGACGCTTTAGTCCA	AAGAACTTGCGTCCAATAGGA	50.0	42.9	58.0	57.3	1934-1953 bp	1.0	68.9