Hotspot

rps15

SSC · 125513-125776 · 264 bp

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Pi 0

MarkerSeek score 21.80

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 264
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 21.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AGTTAAAATGAAATGACTCTTCA	TCTGTAAAATAAAGAAAGGGGA	26.1	31.8	52.3	52.7	329 bp	0.5	20.0
rps15_p2	AGTTAAAATGAAATGACTCTTCA	TCTGTAAAATAAAGAAAGGGGAA	26.1	30.4	52.3	53.5	329 bp	0.5	20.0
rps15_p3	AAGTTAAAATGAAATGACTCTTCA	TCTGTAAAATAAAGAAAGGGGA	25.0	31.8	53.1	52.7	330 bp	0.5	20.0
rps15_p4	AGTTAAAATGAAATGACTCTTCAA	TCTGTAAAATAAAGAAAGGGGA	25.0	31.8	53.1	52.7	329 bp	0.5	20.0
rps15_p5	AGTTAAAATGAAATGACTCTTCAA	TCTGTAAAATAAAGAAAGGGGAA	25.0	30.4	53.1	53.5	329 bp	0.5	20.0