Hotspot

cemA

LSC · 63497-64186 · 690 bp

Back to Scopolia

Pi 0.0087

MarkerSeek score 52.40

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 6
pi: 0.0087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TCGCATAGAGTGTACGAATGG	ACAACTACAAAGTACCAAGCA	47.6	38.1	57.7	55.4	796 bp	1.0	40.8
cemA_p2	GCATAGAGTGTACGAATGGGT	ACAACTACAAAGTACCAAGCA	47.6	38.1	57.5	55.4	794 bp	1.0	40.6
cemA_p3	CGCATAGAGTGTACGAATGGG	ACAACTACAAAGTACCAAGCA	52.4	38.1	58.6	55.4	795 bp	1.0	40.6
cemA_p4	TCGCATAGAGTGTACGAATGG	ACAACTACAAAGTACCAAGCAT	47.6	36.4	57.7	55.9	796 bp	1.0	40.6
cemA_p5	AGAGTGTACGAATGGGTTGA	ACAACTACAAAGTACCAAGCA	45.0	38.1	56.2	55.4	790 bp	1.0	40.6