Hotspot

ndhH-rps15

SSC · 125402-125512 · 111 bp

Back to Scopolia

Pi 0.018

MarkerSeek score 62.90

Primer available yes

Label ndhH-rps15

Feature Metadata

label_name: ndhH-rps15
feature_type: igs
region: SSC
length_bp: 111
variable_sites: 2
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH-rps15_p1	ATGAGTCGAAGAACACCGTG	TTGGGAAAACGTCAACGACT	50.0	45.0	57.9	58.0	302 bp	1.0	79.8
ndhH-rps15_p2	ATGAGTCGAAGAACACCGTG	CGTCAACGACTGCTAGCTTA	50.0	50.0	57.9	57.7	293 bp	1.0	78.0
ndhH-rps15_p3	GATGAGTCGAAGAACACCGT	TTGGGAAAACGTCAACGACT	50.0	45.0	57.7	58.0	303 bp	1.0	77.5
ndhH-rps15_p4	GATGAGTCGAAGAACACCGT	CGTCAACGACTGCTAGCTTA	50.0	50.0	57.7	57.7	294 bp	1.0	75.7
ndhH-rps15_p5	ATGAGTCGAAGAACACCGTG	TGGGAAAACGTCAACGACTG	50.0	50.0	57.9	58.7	301 bp	1.0	74.3