Hotspot

trnL-UAG-ccsA

SSC · 116525-116622 · 98 bp

Back to Scopolia

Pi 0.0306
MarkerSeek score 70.10
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
98
variable_sites
3
pi
0.0306
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 GTGAAATCGGTAGACACGCT ACGATCGAAAAGGAAATGTGAG 50.0 40.9 58.0 57.1 227 bp 1.0 57.6
trnL-UAG-ccsA_p2 GTGAAATCGGTAGACACGCT CGATCGAAAAGGAAATGTGAGT 50.0 40.9 58.0 57.1 226 bp 1.0 57.6
trnL-UAG-ccsA_p3 TGAAATCGGTAGACACGCTG ACGATCGAAAAGGAAATGTGAG 50.0 40.9 58.0 57.1 226 bp 1.0 57.6
trnL-UAG-ccsA_p4 TGAAATCGGTAGACACGCTG CGATCGAAAAGGAAATGTGAGT 50.0 40.9 58.0 57.1 225 bp 1.0 57.6
trnL-UAG-ccsA_p5 TAGACACGCTGCTCTTAGGA CGATCGAAAAGGAAATGTGAGT 50.0 40.9 57.9 57.1 216 bp 1.0 56.7