Hotspot

rpl32-sprA

SSC · 115566-115613 · 48 bp

Back to Scopolia

Pi 0.0208

MarkerSeek score 64.50

Primer available yes

Label rpl32-sprA

Feature Metadata

label_name: rpl32-sprA
feature_type: igs
region: SSC
length_bp: 48
variable_sites: 1
pi: 0.0208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-sprA_p1	AGGAAGGGATATTGGGTAGCA	AGCGAAGAGAGTTCTATTACGT	47.6	40.9	57.9	56.8	239 bp	1.0	45.9
rpl32-sprA_p2	AGCGAAATCTCTTTCTACCGG	AGCGAAGAGAGTTCTATTACGT	47.6	40.9	57.8	56.8	201 bp	1.0	45.2
rpl32-sprA_p3	AGGAAGGGATATTGGGTAGCA	AAGCGAAGAGAGTTCTATTACGT	47.6	39.1	57.9	57.4	240 bp	1.0	43.2
rpl32-sprA_p4	AGCGAAATCTCTTTCTACCGG	AAGCGAAGAGAGTTCTATTACGT	47.6	39.1	57.8	57.4	202 bp	1.0	42.6
rpl32-sprA_p5	AGGAAGGGATATTGGGTAGCA	GCGAAGAGAGTTCTATTACGT	47.6	42.9	57.9	55.3	238 bp	1.0	42.2