Hotspot

ndhF-rpl32

SSC · 114641-115397 · 757 bp

Back to Scopolia

Pi 0.00661
MarkerSeek score 62.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
757
variable_sites
5
pi
0.00661
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGCGAAAACTTTTTGTTGC TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 954 bp 1.0 69.1
ndhF-rpl32_p2 GCGAAAACTTTTTGTTGCCG TGCTACCCAATATCCCTTCCT 45.0 47.6 57.7 57.9 952 bp 1.0 69.1
ndhF-rpl32_p3 GCGAAAACTTTTTGTTGCCG TGATGTCGAAGTGCGTTTTT 45.0 40.0 57.7 56.6 901 bp 1.0 66.4
ndhF-rpl32_p4 GCGAAAACTTTTTGTTGCCG TTGATGTCGAAGTGCGTTTT 45.0 40.0 57.7 56.6 902 bp 1.0 66.4
ndhF-rpl32_p5 GCGAAAACTTTTTGTTGCCG TTTGATGTCGAAGTGCGTTT 45.0 40.0 57.7 56.6 903 bp 1.0 66.4