Hotspot

rps7-rps12

IRb · 99891-99943 · 53 bp

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Pi 0.0189

MarkerSeek score 63.30

Primer available yes

Label rps7-rps12

Feature Metadata

label_name: rps7-rps12
feature_type: igs
region: IRb
length_bp: 53
variable_sites: 1
pi: 0.0189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-rps12_p1	TTTTCTGCAGTACCTCGACG	GGCTTGGAGGGAGATCTTTC	50.0	55.0	57.9	57.7	148 bp	1.0	77.3
rps7-rps12_p2	TTTTCTGCAGTACCTCGACG	TATGTACGGCTTGGAGGGAG	50.0	55.0	57.9	58.6	155 bp	1.0	75.3
rps7-rps12_p3	TTTTCTGCAGTACCTCGACG	GTATGTACGGCTTGGAGGGA	50.0	55.0	57.9	58.9	156 bp	1.0	73.0
rps7-rps12_p4	TTTTCTGCAGTACCTCGACG	GCCGTATTCGATGAAAGTCG	50.0	50.0	57.9	57.1	175 bp	1.0	72.7
rps7-rps12_p5	TTTTCTGCAGTACCTCGACG	GCTTGGAGGGAGATCTTTCA	50.0	50.0	57.9	56.9	147 bp	1.0	71.3