Hotspot

accD-psaI

LSC · 60878-61610 · 733 bp

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Pi 0.00682

MarkerSeek score 62.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 5
pi: 0.00682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGACCAAATTGTACCACGT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.5	57.9	913 bp	1.0	75.4
accD-psaI_p2	TCGACCAAATTGTACCACGT	TGCAATTGCCGGAAAGACTA	45.0	45.0	57.5	57.8	903 bp	1.0	74.7
accD-psaI_p3	TCGACCAAATTGTACCACGT	CCGGAAAGACTAGGCCTACT	45.0	55.0	57.5	58.2	895 bp	1.0	74.4
accD-psaI_p4	TCGACCAAATTGTACCACGT	AGACTAGGCCTACTAACGGC	45.0	55.0	57.5	58.3	889 bp	1.0	73.7
accD-psaI_p5	TCGACCAAATTGTACCACGT	CATTGCAATTGCCGGAAAGA	45.0	45.0	57.5	57.6	906 bp	1.0	73.3