Hotspot

rpoB

LSC · 22810-26022 · 3213 bp

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Pi 0.00124

MarkerSeek score 41.30

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 4
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	CGAGCCCATATCCTTGCTTT	ACCGTCGATAAAAGAAAGGGA	50.0	42.9	58.0	57.0	330 bp	1.0	64.5
rpoB_p2	CGAGCCCATATCCTTGCTTT	CGTCGATAAAAGAAAGGGATGC	50.0	45.5	58.0	57.8	328 bp	1.0	62.7
rpoB_p3	CGAGCCCATATCCTTGCTTT	AACCGTCGATAAAAGAAAGGGA	50.0	40.9	58.0	57.7	331 bp	1.0	62.1
rpoB_p4	CGAGCCCATATCCTTGCTTT	ACCGTCGATAAAAGAAAGGGAT	50.0	40.9	58.0	57.5	330 bp	1.0	60.4
rpoB_p5	CGAGCCCATATCCTTGCTTT	ACCGTCGATAAAAGAAAGGG	50.0	45.0	58.0	55.5	330 bp	1.0	60.1