Hotspot

psbA

LSC · 343-1404 · 1062 bp

Back to Scolopia

Pi 0.00282

MarkerSeek score 60.40

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.00282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAAATTGGCGATTGCTCCT	AGAATTCGTGTGCTTGGGAG	45.0	50.0	57.9	58.2	1173-1174 bp	1.0	77.6
psbA_p2	AGAAATTGGCGATTGCTCCT	AGAGAATTCGTGTGCTTGGG	45.0	50.0	57.9	58.2	1175-1176 bp	1.0	77.6
psbA_p3	AGAAATTGGCGATTGCTCCT	GAGAATTCGTGTGCTTGGGA	45.0	50.0	57.9	58.2	1174-1175 bp	1.0	77.6
psbA_p4	AGAAATTGGCGATTGCTCCT	AATTCGTGTGCTTGGGAGTC	45.0	50.0	57.9	58.5	1171-1172 bp	1.0	75.3
psbA_p5	AGAAATTGGCGATTGCTCCT	GAATTCGTGTGCTTGGGAGT	45.0	50.0	57.9	58.5	1172-1173 bp	1.0	75.3