Hotspot

trnN-GUU-trnR-ACG

IRa · 131815-132449 · 635 bp

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Pi 0.00318
MarkerSeek score 61.50
Primer available yes
Label trnN-GUU-trnR-ACG

Feature Metadata

label_name
trnN-GUU-trnR-ACG
feature_type
igs
region
IRa
length_bp
635
variable_sites
2
pi
0.00318
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-trnR-ACG_p1 CCGTTGTTCCTCAGTAGCTC AACAGATCCAGTGGAGACGA 55.0 50.0 58.0 58.1 806-812 bp 1.0 80.2
trnN-GUU-trnR-ACG_p2 CCGTTGTTCCTCAGTAGCTC CAGAGGATTAGAGCACGTGG 55.0 55.0 58.0 57.8 770-776 bp 1.0 79.1
trnN-GUU-trnR-ACG_p3 GCTGTTAACTGACTGGTCGT AACAGATCCAGTGGAGACGA 50.0 50.0 57.8 58.1 770-776 bp 1.0 79.0
trnN-GUU-trnR-ACG_p4 TGGTCGTAGGTTCGAATCCT AACAGATCCAGTGGAGACGA 50.0 50.0 58.2 58.1 757-763 bp 1.0 79.0
trnN-GUU-trnR-ACG_p5 CTGGTCGTAGGTTCGAATCC AACAGATCCAGTGGAGACGA 55.0 50.0 57.8 58.1 758-764 bp 1.0 78.6