Hotspot

ndhF-trnL-UAG

SSC · 114249-116171 · 1923 bp

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Pi 0.0063
MarkerSeek score 52.60
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1923
variable_sites
4
pi
0.0063
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.33
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CGGAAAAAGTAGGAGTCCCA AGCGTGTCTACCGATTTCAC 50.0 50.0 56.9 58.0 755-2042 bp 1.0 75.0
ndhF-trnL-UAG_p2 CGGAAAAAGTAGGAGTCCCA CAGCGTGTCTACCGATTTCA 50.0 50.0 56.9 58.0 756-2043 bp 1.0 75.0
ndhF-trnL-UAG_p3 CGGAAAAAGTAGGAGTCCCA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 56.9 57.9 765-2052 bp 1.0 74.1
ndhF-trnL-UAG_p4 CGGAAAAAGTAGGAGTCCCA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 56.9 57.8 764-2051 bp 1.0 73.5
ndhF-trnL-UAG_p5 CGGAAAAAGTAGGAGTCCCA CTAAGAGCAGCGTGTCTACC 50.0 55.0 56.9 57.8 763-2050 bp 1.0 73.5