Hotspot

trnR-ACG-trnN-GUU

IRb · 109098-109732 · 635 bp

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Pi 0.00318
MarkerSeek score 61.50
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
635
variable_sites
2
pi
0.00318
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 AACAGATCCAGTGGAGACGA CCGTTGTTCCTCAGTAGCTC 50.0 55.0 58.1 58.0 806-812 bp 1.0 80.2
trnR-ACG-trnN-GUU_p2 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 770-776 bp 1.0 79.1
trnR-ACG-trnN-GUU_p3 AACAGATCCAGTGGAGACGA GCTGTTAACTGACTGGTCGT 50.0 50.0 58.1 57.8 770-776 bp 1.0 79.0
trnR-ACG-trnN-GUU_p4 AACAGATCCAGTGGAGACGA TGGTCGTAGGTTCGAATCCT 50.0 50.0 58.1 58.2 757-763 bp 1.0 79.0
trnR-ACG-trnN-GUU_p5 AACAGATCCAGTGGAGACGA CTGGTCGTAGGTTCGAATCC 50.0 55.0 58.1 57.8 758-764 bp 1.0 78.6